pyFuRNAce is a design engine for RNA origami. It offers an integrated, user-friendly open-source platform for RNA origami design — a web-based application that makes rational RNA structure design as intuitive and modular as current tools for DNA origami and protein design. PyFuRNAce enables users to design complete RNA nanostructures — from motif selection and blueprint generation to sequence design, structural optimization, and primer planning — all within a single graphical interface. We also provide advanced scripting functionality for users who wish to programmatically design and customize complex assemblies.

Web-app accessible at pyfurnace.de; Github repository

Documentation including video demo can be found here.

When using pyFuRNAce please cite: Monari, L., Braun, I., Poppleton, E., & Göpfrich, K. (2025). PyFuRNAce: An integrated design engine for RNA origami. bioRxiv, 2025-04. DOI: 10.1101/2025.04.17.647389 

RingAScientist is a webplatform designed to bring the latest science into the classroom – live by webvideoconferencing. Join our growing community!

Find out more at ring-a-scientist.org

An explanatory video can be found here.

We share video protocols and animations on our YouTube channel. Feel free to reuse in lectures or elsewhere.

Browse on YouTube

We share our code on GitHub. Amongst others, you find tools for RNA design, 3D surface reconstruction from confocal stacks or protocols we use to run our pipetting robot.

Check out our GitHub repository